| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059310 |
| Chromosome: | chromosome 10 |
| Location: | 3069689 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441550 | MAM3B | (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCTCGCCACCATGGCGCCGTCGTCGGG |
| Internal bar code: | GTCTACTCCGTCCTGCCGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 99.3155 |
| LEAP-Seq n confirming: | 3482 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGGCTGATAGCTCTGGG |
| Suggested primer 2: | GGTTGGAGTTGGAGGTCTCA |