| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059330 |
| Chromosome: | chromosome 15 |
| Location: | 179839 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635100 | (1 of 2) IPR000104//IPR010402 - Antifreeze protein, type I // CCT domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTACGCTAACTAGTCAGCAGCCCAGCA |
| Internal bar code: | TTGCGCTCACAGTACCTGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 99.6721 |
| LEAP-Seq n confirming: | 2432 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCAGCAACTGCAACAAC |
| Suggested primer 2: | TTGCTATGCTTTGCAACTGG |