Insertion junction: LMJ.RY0402.059331_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.059331
Chromosome:chromosome 6
Location:1989606
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g264050 (1 of 5) PF04366 - Las17-binding protein actin regulator (Ysc84) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACACTCGCGCGCACCCTCGCCGTCCAGC
Internal bar code:CCATTGTGTAGCAAATAACGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:381
LEAP-Seq percent confirming:99.7876
LEAP-Seq n confirming:9865
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGCTGTTGGCATCACTGC
Suggested primer 2:CCCCAGTTACCGTAATCCCT