| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059336 |
| Chromosome: | chromosome 9 |
| Location: | 7684119 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415500 | TCA1 | Translation factor for chloroplast petA RNA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACGTGGAGCTGGAGGCGCTCATCTGGG |
| Internal bar code: | CGCGCTTAGATAGGCAATCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 81.2155 |
| LEAP-Seq n confirming: | 147 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGTGTATGCGACTCTGG |
| Suggested primer 2: | CGCCAAACCAAAAGAACAAT |