Insertion junction: LMJ.RY0402.059375_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.059375
Chromosome:chromosome 3
Location:1504941
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g152000 ABA3,MCS1 (1 of 2) K15631 - molybdenum cofactor sulfurtransferase (ABA3); Molybdenum cofactor sulfurase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGTGCGAGAATCGCGAGCAAGAGCGCAC
Internal bar code:TCCATCACGGGGCCGTTTGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:99.6184
LEAP-Seq n confirming:4438
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTGATGATTGGTGGGCAT
Suggested primer 2:AGCTCATGCCGCTCTGTAAT