Insertion junction: LMJ.RY0402.059376_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.059376
Chromosome:chromosome 16
Location:316893
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g694207 (1 of 1) IPR000945//IPR001810 - Dopamine beta-hydroxylase-related // F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTCCCTGCACGCCCTCCGCCCCACGAG
Internal bar code:GGGCACAGGGGAAATATGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:193
LEAP-Seq percent confirming:99.6606
LEAP-Seq n confirming:1762
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTCGACGAGGTCTGTCA
Suggested primer 2:CGCTCCGTTTTGTTTCTCTC