| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059404 |
| Chromosome: | chromosome 12 |
| Location: | 2601913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505100 | (1 of 1) K13281 - UV DNA damage endonuclease (uvsE, UVE1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCACCCCTCCGAGTTTACAAAGATTGCC |
| Internal bar code: | GGCCCGCGGCGCTACCGAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 858 |
| LEAP-Seq percent confirming: | 97.6182 |
| LEAP-Seq n confirming: | 5369 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCTCGCTCTATTGCAT |
| Suggested primer 2: | AGTAGCAGTTGGATGGGTGG |