| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059433 |
| Chromosome: | chromosome_12 |
| Location: | 6474006 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g538300 | LF5,FAP247 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACGCGGCTTCGGGTGCTAGTCTTTGGCTG |
| Internal bar code: | GCATCATCGTGTATGCGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 27.8291 |
| LEAP-Seq n confirming: | 873 |
| LEAP-Seq n nonconfirming: | 2264 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTCCGTTCCACGTACTCG |
| Suggested primer 2: | TGTAGGGGGAGTACACGGAG |