Insertion junction: LMJ.RY0402.059437_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.059437
Chromosome:chromosome 1
Location:6814345
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g049132 (1 of 1) PTHR23097//PTHR23097:SF105 - TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCGTCAATCCAATCCCGCTCCACGCACC
Internal bar code:ACGGGGCGCGCGCTCGTGTCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:775
LEAP-Seq percent confirming:99.8212
LEAP-Seq n confirming:2792
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCACACATGTCATCTACGGG
Suggested primer 2:GAACATGCAGGAGAACAGCA