| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059440 |
| Chromosome: | chromosome 12 |
| Location: | 535025 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492950 | NSG5,DIV15,RIR1 | (1 of 1) K10807 - ribonucleoside-diphosphate reductase subunit M1 (RRM1); Ribonucleoside-diphosphate reductase R1 subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCAAAGAGTGAATGAATTGTGGAAGGGA |
| Internal bar code: | TGTCGTATCGTTGGGGCGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 88.8625 |
| LEAP-Seq n confirming: | 2242 |
| LEAP-Seq n nonconfirming: | 281 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAGTGCAGTAAGGCTAC |
| Suggested primer 2: | CGCTGTCCAAGAAGAAGACC |