| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059448 |
| Chromosome: | chromosome 12 |
| Location: | 5252756 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACAAACTCTGGGTCTGTCGCGGAGAA |
| Internal bar code: | GCGGTGGTGCACATAAGGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 98.9149 |
| LEAP-Seq n confirming: | 4102 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTCCACCTCGTGTTGTG |
| Suggested primer 2: | AAAACAGCAATTGGTCCAGG |