| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059452 |
| Chromosome: | chromosome 17 |
| Location: | 3749365 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACCGGAGGCGGGGCCTGAGCCGGAGG |
| Internal bar code: | TGGAGTACGCCAACGCGGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 89.6024 |
| LEAP-Seq n confirming: | 586 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTAGCAGCAGCAGTAGCG |
| Suggested primer 2: | TCCTCCTGCTCTTGCTCATT |