| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059455 |
| Chromosome: | scaffold 24 |
| Location: | 50486 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755447 | (1 of 1) K12863 - protein CWC15 (CWC15) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGGCCACGGCGCTGGCCCAACCCCCAA |
| Internal bar code: | CGGAAAGGTTGCGATCCACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 98.8539 |
| LEAP-Seq n confirming: | 345 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGCAGAGAAGGGTAGC |
| Suggested primer 2: | CCCCTCAGTCTTTTATGCCA |