| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059463 |
| Chromosome: | chromosome 1 |
| Location: | 6551272 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046800 | MSP1 | Membrane selenoprotein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTTATCGATTTGATGAGCTGGTACAGC |
| Internal bar code: | GGCCACGTGTTCACGGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 98.6997 |
| LEAP-Seq n confirming: | 1594 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTCCGACCTACTCCCTT |
| Suggested primer 2: | ACTGCGCGAAAGTTATTGCT |