| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059465 |
| Chromosome: | chromosome 11 |
| Location: | 903789 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467656 | (1 of 9) IPR007112//IPR009009 - Expansin/pollen allergen, DPBB domain // RlpA-like double-psi beta-barrel domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCACAGTCGTGTCTCCTGCGGTCTCCG |
| Internal bar code: | CACCTTCCGGCGCCTTCTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 98.601 |
| LEAP-Seq n confirming: | 3665 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGTGACGTAGATTGCAT |
| Suggested primer 2: | ACACGGGACAGTTTGCCTAC |