| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059469 |
| Chromosome: | chromosome 3 |
| Location: | 5269553 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183350 | SRH7 | SNF2-related DNA/RNA helicase; (1 of 1) PTHR10799//PTHR10799:SF654 - SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCCGCAGCACGTCAACAGCTTGGGCT |
| Internal bar code: | GCTACTTTTAAATTTATCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 85.2659 |
| LEAP-Seq n confirming: | 5949 |
| LEAP-Seq n nonconfirming: | 1028 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGTGCGATGTTCTCCAA |
| Suggested primer 2: | GAACAGGTCTTTCAGCTCCG |