| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059500 |
| Chromosome: | chromosome 4 |
| Location: | 3630589 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228650 | (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGAATGTAACGCTTGCCCCCATCACGC |
| Internal bar code: | TTACTCGGTCCTTAGCGCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 98.3878 |
| LEAP-Seq n confirming: | 2380 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTGTGGACAGAGGTGT |
| Suggested primer 2: | CCCTTTCACGGGCATAGTTA |