Insertion junction: LMJ.RY0402.059500_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.059500
Chromosome:chromosome 4
Location:3630619
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g228650 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAAAACGCACACGTGTAATACAGACACA
Internal bar code:AGGTGTATAGCGCCCCCTGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:193
LEAP-Seq percent confirming:1.06318
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:6514
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGTGTGGACAGAGGTGT
Suggested primer 2:CCCTTTCACGGGCATAGTTA