| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059523 |
| Chromosome: | chromosome 10 |
| Location: | 2184219 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433700 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACGATGCGCTCACCATAATCCAATGG |
| Internal bar code: | AAGGCAGAGTGTGAACCCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGTGGCTCATCTGAAA |
| Suggested primer 2: | CTGCCCCGAATTAACCTGTA |