| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059546 |
| Chromosome: | chromosome 8 |
| Location: | 388339 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358575 | TRP7 | Transient receptor potential ion channel protein; (1 of 1) IPR000104//IPR002110//IPR005821//IPR020683 - Antifreeze protein, type I // Ankyrin repeat // Ion transport domain // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTTCGACGCGGCCTTCCACTCGACGCC |
| Internal bar code: | GAATCGGCTGGGAGGTTTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 99.5396 |
| LEAP-Seq n confirming: | 1946 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCACGTCGGAAAGTCGT |
| Suggested primer 2: | CCTGCGATCTGTCGTACTCA |