| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059569 |
| Chromosome: | chromosome 12 |
| Location: | 714464 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTCCGCCCGCTCCCCTCCATCCTTCAA |
| Internal bar code: | TGCGAGTTACGCGTCTGAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 89.8397 |
| LEAP-Seq n confirming: | 13396 |
| LEAP-Seq n nonconfirming: | 1515 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTCTCCCGGTTATACAT |
| Suggested primer 2: | GTGGAAGGAGCAGTCCAGAG |