| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059583 |
| Chromosome: | chromosome 9 |
| Location: | 4775402 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397327 | (1 of 1) PTHR11139:SF68 - DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCCGCCGCAGCGACCCCCGCGCATTGA |
| Internal bar code: | CTCCACAGCGATCCGAGTTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 60 |
| LEAP-Seq percent confirming: | 88.1919 |
| LEAP-Seq n confirming: | 239 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGACGGTAGCCTGGTGAT |
| Suggested primer 2: | TCCGTCCAGATAAGGACCAC |