| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059593 |
| Chromosome: | chromosome 9 |
| Location: | 524218 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404050 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACCTAGTTTGCCTTTGTGGTCGCAGCC |
| Internal bar code: | GCACCTTGCCGTTCCTTGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 96.3372 |
| LEAP-Seq n confirming: | 8732 |
| LEAP-Seq n nonconfirming: | 332 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCTCCGACCTTCTACCC |
| Suggested primer 2: | TCGACATAATCCACGCATGT |