| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059603 |
| Chromosome: | chromosome 9 |
| Location: | 6804268 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409650 | (1 of 18) PF12906 - RING-variant domain (RINGv) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATCCTTGGGTGAAATCTTGGGGCAGCGG |
| Internal bar code: | CCTCGGCCAGAACGAAGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 95.625 |
| LEAP-Seq n confirming: | 12852 |
| LEAP-Seq n nonconfirming: | 588 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGAACCTTCAAACGGCT |
| Suggested primer 2: | TGCGTTATGGCATTTGTGTT |