| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059607 |
| Chromosome: | chromosome 7 |
| Location: | 743913 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317800 | (1 of 1) PF04091 - Exocyst complex subunit Sec15-like (Sec15) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCGGCGCCAATGCAAGCCTTGTCCTGG |
| Internal bar code: | CAGTATTGAGTGGTTTTATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 70.5137 |
| LEAP-Seq n confirming: | 2951 |
| LEAP-Seq n nonconfirming: | 1234 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGCCAAACGACTCTTAG |
| Suggested primer 2: | GCTCCGCTCATTTTAGTTGC |