| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059615 |
| Chromosome: | chromosome 1 |
| Location: | 654403 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003524 | (1 of 1) PF13499//PF14531 - EF-hand domain pair (EF-hand_7) // Kinase-like (Kinase-like) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTCGGAAGCGCTAGGAAAGCAGCTAGG |
| Internal bar code: | GCACCTAATGCCCCGTTCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1151 |
| LEAP-Seq percent confirming: | 99.5982 |
| LEAP-Seq n confirming: | 2231 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGCGCTTGATCTATTG |
| Suggested primer 2: | CCAACCATGCCTTTCGTAAT |