| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059628 |
| Chromosome: | chromosome 3 |
| Location: | 5361656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184350 | (1 of 1) K13094 - RNA-binding protein 5/10 (RBM5_10) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACGCCGGGCACCGCGGCGGCCTCCAG |
| Internal bar code: | TCCCTTCGAGATTCGGGCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 4.85604 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 2214 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGAAGTTCGCAAGGCTC |
| Suggested primer 2: | ACGTAGGGGAGTCCGAGAGT |