| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059629 |
| Chromosome: | chromosome 12 |
| Location: | 1422317 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487950 | (1 of 2) IPR000742//IPR017853 - EGF-like domain // Glycoside hydrolase superfamily | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGCCCTGTGGAGAACTCCGTGTTGCGG |
| Internal bar code: | TGTCCGTCATTTTCATCTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 99.7517 |
| LEAP-Seq n confirming: | 6427 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGACGTGGAGTACAAAA |
| Suggested primer 2: | GCAGCCTACAACATGCTCAA |