| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059632 |
| Chromosome: | chromosome_9 |
| Location: | 2766503 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g389150 | FAP93 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGACATTGACATGGAGGCGGAGGCGGCGC |
| Internal bar code: | CACCTGCGCGAGGCGCCTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.665 |
| LEAP-Seq n confirming: | 595 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAAGGAGGAGGAGGAGG |
| Suggested primer 2: | AGTGCATAAAACCACCAGGC |