| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059641 |
| Chromosome: | chromosome 9 |
| Location: | 7912893 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417200 | (1 of 1) K15030 - translation initiation factor 3 subunit M (EIF3M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTGGAGCATGTAAGTGTGCGGGCCACA |
| Internal bar code: | GGAGGGCACAGTGGCGCTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 99.4242 |
| LEAP-Seq n confirming: | 6044 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTGCTTTTAGCGAGGCAA |
| Suggested primer 2: | CAGCTGCTCACTGTCTTTGC |