| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059674 |
| Chromosome: | chromosome 7 |
| Location: | 2414777 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328800 | NSG13 | (1 of 8) PF00514 - Armadillo/beta-catenin-like repeat (Arm); Protein expressed during nitrogen-starved gametogenesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTCAGTGTTGTGGTCTGCCCCGCCAAG |
| Internal bar code: | CAAGCATGCGGGGACCGTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 97.464 |
| LEAP-Seq n confirming: | 5342 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTGTGTACCTTCATGCT |
| Suggested primer 2: | GACCCTTACTAACCGGCTCC |