| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059682 |
| Chromosome: | chromosome 1 |
| Location: | 3161283 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019750 | (1 of 1) PTHR10871:SF4 - DNA TOPOISOMERASE 6 SUBUNIT B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGGAGGTTGAAGACATATAAGAAGGG |
| Internal bar code: | ATTGGGATTGGCTCAGTATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 99.3447 |
| LEAP-Seq n confirming: | 758 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCTGCAGAAGGAGTTCG |
| Suggested primer 2: | GTTGGCGGTGTGTGTGTTAC |