| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059701 |
| Chromosome: | chromosome_9 |
| Location: | 4731662 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g397142 | TRP15 | Transient receptor potential ion channel protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACACATGCACCCATGCGTTCCCACCTGTGT |
| Internal bar code: | TTTCGCGACGATGGCGACGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 99.7449 |
| LEAP-Seq n confirming: | 5866 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCTGTCGCTGTTCTCAT |
| Suggested primer 2: | ACCCTTTAGTTTGGGCTGGT |