| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059736 |
| Chromosome: | chromosome 1 |
| Location: | 4805453 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033200 | (1 of 3) IPR001510 - Zinc finger, PARP-type | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTATGGGTCCAGGCCTGTCCAGGAAAGC |
| Internal bar code: | GTGCAGGGGACTGGCGCCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 99.8751 |
| LEAP-Seq n confirming: | 3998 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCACCTCCTGCTACTGCT |
| Suggested primer 2: | TGACATTCACGAAGTCGAGC |