| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059738 |
| Chromosome: | chromosome 17 |
| Location: | 3030854 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720550 | (1 of 1) PTHR34202:SF1 - UPF0548 PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACTCTCTAGCAAGCCGTACCTTTGCGA |
| Internal bar code: | AAGTGCGCGAGACAAAGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 97.8326 |
| LEAP-Seq n confirming: | 2618 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTGGTACCTGCCAAACT |
| Suggested primer 2: | CCTGTAGTGCGGGCATTAGT |