| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059738 |
| Chromosome: | chromosome 17 |
| Location: | 3030857 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720550 | (1 of 1) PTHR34202:SF1 - UPF0548 PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAACACAGCACATGAGCGGAGAGGTGT |
| Internal bar code: | GGGCGACTCCTGCGGGCATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 93.8034 |
| LEAP-Seq n confirming: | 6040 |
| LEAP-Seq n nonconfirming: | 399 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTAGTGCGGGCATTAGT |
| Suggested primer 2: | CCACTGGTACCTGCCAAACT |