Insertion junction: LMJ.RY0402.059741_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.059741
Chromosome:chromosome 17
Location:2675719
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g717800 NUP93 Nucleoporin 93; (1 of 1) K14309 - nuclear pore complex protein Nup93 (NUP93, NIC96) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCTCACACCTGCTGTGCCATTTTGACTG
Internal bar code:CCGTGGTGCACCGACTAGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:510
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:154
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCAGACTAAACCAAACCA
Suggested primer 2:GCGAGTGTGAGAGGGAAAAC