| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059761 |
| Chromosome: | chromosome 9 |
| Location: | 6469950 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407250 | FAP270 | (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED; Flagellar Associated Protein 270 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCGAGTTCCGCAGCCCAGTGCAGGGG |
| Internal bar code: | AAATAGCCAAACGATAGCAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 99.3893 |
| LEAP-Seq n confirming: | 6998 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTCACTTTTGTCGAGCC |
| Suggested primer 2: | GATGCCCCTGTCTTTGTTGT |