| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059765 |
| Chromosome: | chromosome 3 |
| Location: | 6586505 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196200 | (1 of 2) PTHR17630//PTHR17630:SF32 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGAGCCATCACCCTGGGCTTCAAGTGA |
| Internal bar code: | TAGCAAAGCCCACGCTAAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 51 |
| LEAP-Seq percent confirming: | 99.0783 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGGTGACAGGTAAGCAG |
| Suggested primer 2: | AAATCCATTGCGAACGAAAC |