| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059776 |
| Chromosome: | chromosome 10 |
| Location: | 3436407 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCGGGCATTGCCGGACACGCACCCGC |
| Internal bar code: | ACGTTATCCATTCAAGACAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAAACGATTCACGGACGC |
| Suggested primer 2: | GCTGTTCACTGCCAAGACCT |