| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059806 |
| Chromosome: | chromosome 1 |
| Location: | 970550 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005350 | TNP3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGATGCAAGTGCTGCTGTTGTGCGGTAG |
| Internal bar code: | GGGTGCTCTGGGGAATGGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1010 |
| LEAP-Seq percent confirming: | 98.0453 |
| LEAP-Seq n confirming: | 2859 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCACTGGCTTTCTTGTGC |
| Suggested primer 2: | ACACGTATAGTTGCAGGGGC |