Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.059807 |
Chromosome: | chromosome 2 |
Location: | 781332 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g078804 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCACTCCCTACTATACCTGCAAGTCCTG |
Internal bar code: | CAGGCGGCACTTAGTAGGACAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 810 |
LEAP-Seq percent confirming: | 99.6743 |
LEAP-Seq n confirming: | 306 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCTCTGGTGAAGACGCACA |
Suggested primer 2: | AAGCAGGAAGAAAAGGGCTC |