| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059815 |
| Chromosome: | chromosome 15 |
| Location: | 842486 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATGAATGTAACCCCCTCCTTCCCAACC |
| Internal bar code: | TCACAGCGGAGCCCATTGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 94.5055 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCAACTCTCCCAACCAC |
| Suggested primer 2: | TGTCTTGTATCTTGCCGACG |