Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.059841 |
Chromosome: | chromosome_9 |
Location: | 5184098 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g399215 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAAGTTTGAACAACTCACGCCCCTCCCCAT |
Internal bar code: | ATTTTCAGGGGCTCCGCCAGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 937 |
LEAP-Seq percent confirming: | 90.3083 |
LEAP-Seq n confirming: | 8144 |
LEAP-Seq n nonconfirming: | 874 |
LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAGCCACATCCAACACCTC |
Suggested primer 2: | TTCGACTAGCTCTCCTCCCA |