| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059882 |
| Chromosome: | chromosome 12 |
| Location: | 5454564 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530550 | KDG2 | (1 of 1) 2.7.1.91 - Sphinganine kinase / Dihydrosphingosine kinase; Diacylglycerol kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAATGGATGGACCCCGCTCTCTCCCTT |
| Internal bar code: | TCGCTCCTTAAGATTAACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTCCAACAGGAATACAT |
| Suggested primer 2: | TGCGGTATGTTTCAGTTCCA |