| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059883 |
| Chromosome: | chromosome_13 |
| Location: | 1780939 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g574900 | FAP228,GTR2 | Glycosyltransferase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGAACCAGGACAACGTGCTGGCCGAGTGC |
| Internal bar code: | GGGTTCCCTATAAGGGTTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1040 |
| LEAP-Seq percent confirming: | 99.8096 |
| LEAP-Seq n confirming: | 2097 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGCATATCGCTCATGT |
| Suggested primer 2: | CTAGTTCGAGTTTCCAGCCG |