| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059884 |
| Chromosome: | chromosome_9 |
| Location: | 3096780 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g386850 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCACAGCAGGGCACGCGCAGCTGCTGTCA |
| Internal bar code: | AGAGCTTCCAAAGTCAATTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 99.1708 |
| LEAP-Seq n confirming: | 2392 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACAGGAACAGCGAAGGA |
| Suggested primer 2: | GCGCACTCTGTTTCACACAT |