| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059885 |
| Chromosome: | chromosome 8 |
| Location: | 4048246 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379350 | TPT10,TPT | phosphate/phosphoenolpyruvate translocator; (1 of 2) PTHR11132:SF70 - PHOSPHOENOLPYRUVATE/PHOSPHATE TRANSLOCATOR 1, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGTACGGCTGCCCCCTGCCTCCCCCAA |
| Internal bar code: | AACAGCACTTGGGCCGGCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 98.7791 |
| LEAP-Seq n confirming: | 6715 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAATGTACCCCCTCCCCTC |
| Suggested primer 2: | AGCAGTGCACTCATACGCTG |