| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059904 |
| Chromosome: | chromosome 16 |
| Location: | 6449035 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673300 | Chlamydomonas-specific family W protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATCAACACGTGCGCACACTGTCATCAC |
| Internal bar code: | CGGGGGGCGGGACCCCAGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2721 |
| LEAP-Seq percent confirming: | 97.9352 |
| LEAP-Seq n confirming: | 5502 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCCAAGTCGAATGTA |
| Suggested primer 2: | TGGGACAAGATCCACACAAA |