| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059908 |
| Chromosome: | chromosome 13 |
| Location: | 4719969 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604650 | (1 of 1) PTHR10804//PTHR10804:SF11 - PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P // PROLIFERATION-ASSOCIATED PROTEIN 2G4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGATAGCCCCAACTAAAGCCCCCTGCT |
| Internal bar code: | GCCGGCGAGGCGAATGGCCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 96.3866 |
| LEAP-Seq n confirming: | 1067 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCATCCAGAGCTCCATTG |
| Suggested primer 2: | GTATGCGCTCTCACCTGTCA |